Production, one-step purification, and characterization of a keratinolytic protease from Serratia marcescens P3

Production and Characterization of Protease from Serratia marcescens

Extracellular protease produced by Serratia marcescens isolated from the gut of termite (Centrocestus formosanus) was investigated in this study. The effect of temperature, pH, carbon and nitrogen sources on protease production was examined. The extracellular protease was separated from the culture supernatant of the organism through precipitation with ammonium sulfate. The protease was purifie...

Purification and partial characterization of a bacteriocin from Serratia marcescens.

Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m urea. In the absence of these agents, the bacteriocin associates into aggregate...

A Novel Production and Characterization of Serratiopeptidase from Serratia Marcescens

Purification and characterization of a Serratia marcescens metalloprotease.

An extracellular, nonelastolytic, neutral metalloprotease of Serratia marcescens was purified by sequential ammonium sulfate precipitation, hydroxyapatite adsorption chromatography, flat-bed isoelectric focusing, and Sephadex G-100 gel filtration. The protease preparation had a 280/260 nm absorbance ratio of 1.8, was free of detectable amounts of endotoxin, carbohydrate, phosphorus, and other k...

Purification and partial characterization of psychrotrophic Serratia marcescens lipase.

Serratia marcescens isolated from raw milk was found to produce extracellular lipase. The growth of this organism could contribute to flavor defects in milk and dairy products. Serratia marcescens was streaked onto spirit blue agar medium, and lipolytic activity was detected after 6 h at 30 degrees C and after 12 h at 6 degrees C. The extracellular crude lipase was collected after inoculation o...